Species |
Gene ID |
Accession |
Source |
Length |
MW |
Tag |
Mouse |
16198 |
P15247 |
E. coli |
126aa |
14.2kDa |
- |
About this Antibody |
Name |
Recombinant Murine IL-9 (Recombinant Murine Interleukin-9; rMuIL-9);Recombinant Mouse IL-9 ; 重组小鼠白细胞介素9 |
Synonyms |
Cytokine P40; HP40; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; P40; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40 |
Purity |
>97% by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine TS1 cells is less than 0.02ng/ml, corresponding to a specific activity of >5.0×107IU/mg. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
Endotoxin |
Less than 0.1EU/μg of rMuIL-9 as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category |
Cytokine |
Background |
Murine interleukin-9 (IL-9) was originally identified as T cell-derived T cell growth factor III/P40 that could support the long term growth of certain murine T helper clones in the absence of antigen or antigen-presenting cells. Human IL-9 was independently cloned as a novel growth factor that is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Murine IL-9 shares 56% amino acid sequence identity with human IL-9. Although murine IL-9 is active on human cells, human IL-9 is not active on murine cells. |
Amino Acid Sequence |
QRCSTTWGIR DTNYLIENLK DDPPSKCSCS GNVTSCLCLS VPTDDCTTPC YREGLLQLTN ATQKSRLLPV FHRVKRIVEV LKNITCPSFS CEKPCNQTMA GNTLSFLKSL LGTFQKTEMQ RQKSRP |
包装清单:
产品编号 |
产品名称 |
包装 |
P5934-100μg |
Recombinant Murine IL-9 |
100μg |
--- |
说明书 |
1份 |
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。
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